Herbal medicaments have been practiced since antiquity as natural remedies,
for the management of acute and chronic ailments. Diabetic wound healing activity of
Securinega leucopyrus (Willd.) Muell has been reported in several case studies from India
and Sri Lanka. Objectives: In vitro antimicrobial activity of different formulations of
Securinega leucopyrus oil was investigated against clinically significant bacterial and fungal
strains. Material and Methods: Five different samples of S. leucopyrus oil and ointments
were blinded and studied with standard control Ampicillin (against S. aureus, E. coli, P.
aeruginosa, K. pnumonie) and Fluconazole (against C. albicans, C. glabrata, C. tropicalis
and Aspergillus flavus) using agar disc diffusion method and Minimum Inhibitory
Concentration (MIC) values were estimated using broth dilution method. Results: A zone
of inhibition (ZOI) of 16 mm was achieved at 14 /ml, 16 /ml and 12 /ml at MIC of dry drug-
based S. Leucopyrus. oils against C. albicans, C. glabrata, C. tropicalis, respectively,
whereas no sample showed antifungal activity against Aspergillus flavus. Fresh and Dry
drug based Thumari oils represented antibacterial activity with a ZOI of 21 mm achieved at
an MIC of 26 /ml against Staphylococcus aureus. Both the fresh and dry drug base ointments did not produce any antimicrobial action against the tested strains. Conclusion:
Thumari oils presented a significant antifungal action against all three Candida species and
antibacterial action against the gram-positive S. aureus strains.
Key words: Securinega leucopyrus, antibacterial, antifungal, wound healing
