Development of wound healing hydrogels using bio synthesized silver nano particles and its antibacterial activity of Major Wound pathogens

Authors

Reetha K, Suganya K
Department of Microbiology, Sri Ramakrishna College of Arts and Science for Women, Coimbatore, TamilNadu-641044, India.

Karkuvelraja R
Microbial Biodiversity and Bioenergy Lab, Department of Microbiology, Bharathidasan University, Tiruchirapalli-620024, India.

Sanjay Prasad S
Department of Microbiology, CMS College of Science and Commerce, Coimbatore-641049, Tamil Nadu, India.

Abstract

In this present study, biosynthesis of AgNp’s from methanolic extracts of H. Colorata and its wound healing activity was documented. The synthesis of AgNp’s was done by treating AgNO3 solution with an aqueous extract of H. colorata. The production of AgNp’s was confirmed by a color change of the solution from clear to brown color. The reduced AgNp’s were characterized by Scanning Electron Microscope (SEM), UV–vis spectroscopy. From UV analysis peak was observed at 415nm and spherical shaped AgNp’s were observed. The antibacterial activity and Minimum inhibitory Concentration (MIC) of the silver nanoparticles were determined. The results suggest that biosynthesized AgNp’s from aqueous extracts ofH.coloratashowed a significant antibacterial activity against wound pathogens.