Fabrication and Analysis of Nanofibrous scaffolds from Plumeria alba for biomedical application

Antibiotic resistance is rising to dangerously high levels and new resistance mechanisms are emerging and spreading globally, threatening our ability to treat common infectious diseases. nanotechnology is one of the fast growing research areas in the field of biomedicine. The current studies discuss the synthesis of hydroxyapatite nanoparticles with various herbal extracts by wet synthesis technique, characterization of developed nanoparticles and final fabrication of nanofibrous scaffolds with PHB as the polymer. Fabrication of nanofibrous scaffolds from Plumeria alba latex serum extract was developed along with polymer polyhydroxy butyrate. Poly-β-hydroxybutyrate (PHB) is a biopolymer that belongs to polyester produced from micro-organisms. Polyhydroxy butyrate along with developed hydroxyapatite nanoparticles as three-dimensional scaffolds was developed by electrospinning procedure. The developed nanofibrous scaffolds were characterized for thickness, tensile strength, water contact angle (wetting ability), FTIR and SEM. Nanofibrous scaffolds of Plumeria alba were evaluated for cell proliferation by MTT cytotoxicity test against 3T3-L1 cell line on different concentrations. Nanofibrous scaffold developed from Plumeria alba with hydroxyapatite nanoparticles has potent biomedical application for wound dressing and tissue regeneration.