Analysis of Proximate composition, Phytochemical profiling, Antimicrobial and Antioxidant activity of methanolic extracts of Diospyros melanoxylon Roxb. fruits

D. melanoxylon displays exceptional qualities as a naturally occurring antioxidant and antimicrobial agent. The investigation focuses on phytochemical profiles, proximate composition, antioxidant and antimicrobial activities of methanolic extract of Diospyros melanoxylon fruits. Methanolic extract of the fruits are processed for the determination of total flavonoid and total phenolic content, proximate composition, antioxidant and antimicrobial activity. Different biochemical methods are used for performing these experiment. The determination of total flavonoid content (TFC) and total phenolic content (TPC) is 40.6±4.1μg/mg and 211±5.03 μg/mg respectively. The amount of β-carotene and lycopene is 0.0924± 0.01μg/mg and 0.035± 0.005μg/mg respectively. The amount of total moisture content, total protein content, total carbohydrate content, total fibre content and total lipid content is 85.45± 0.37 g/100g, 6.81 ±0.29 g/100g, 25.78± 0.37 g/100g, 3.13± 0.18 g/100g and 2.39 ±0.04 g/100g, respectively. The IC 50 values of ABTS and DPPH are 74.65 ±4.38 μg/ml and 59.14 ±1.08 μg/ml respectively. Further, antibacterial activity is studied by the agar well diffusion method using two different bacteria Pseudomonas aeruginosa (MTCC 741) and Staphylococcus aureus (MTCC 87). Antifungal activity is studied by the disc diffusion method against the fungi Candida albicans (MTCC 227).The MIC values of Staphylococcus aureus and Pseudomonas aeruginosa are0.45 mg/ml and 0.30 mg/ml respectively. Fruit extract does not showed antifungal activity. Conducted LC/MS analysis indicated the existence of 7 compounds. Amongst them main is Vallinic acid. Thus, the fruits of Diospyros melanoxylon can be successfully used as constituent in health to reduce oxidative stress.