Animal Studies on Venom Neutralization potential of Metal-Herbal (Copper-Leucas zeylanica) Nanocomposites (MHNC) Against Naja naja and Bungarus Caeruleus Venoms


Pooja S Dev, Research scholar
Nehru arts and science college, Coimbatore , Tamil Nadu, India.
Dr. S. Meenatchisundaram, Associate Professor
Department of Microbiology, Shri Nehru Maha Vidyalaya College of Arts & Science, Coimbatore, Tamil Nadu, India.


Objectives: The present study focuses on development of novel Metal-Herbal Nano composite (MHNC) formulation against Naja naja and Bungarus Caeruleus venoms on animal models. Methods: The venoms were procured from Irula snake catchers society in lyophilised forms. Herbal-metal nanocomposites were prepared in 1:1 combinations. Single strength concentration of Leucas zeylanica extracts and copper oxide metal nanoparticles was used to attain these ratio’s. In vivo venom neutralization analysis was carried out in Swiss albino mice. The LD50 and ED50 of the MHNC were determined. Results: The LD50 of the Naja naja venom was observed to be 0.19μg/g and 0.174μg/g for Bungarus Caeruleus venom. The ED50 of the MHNC against Naja naja venom was observed to be 14.22mg and 63.39mg for Bungarus Caeruleus venom. Conclusion: The MHNC developed in this study has significant venom neutralization potential against Naja naja and Bungarus Caeruleus venoms. Therefore, the MHNC can be used for development of anti-venom drugs.