Anti-Psoriatic Activity of Indigofera Tinctoria Leaves Extract on Staphylococcus Aureus Embedded Hacat Cells: A Systematic Approach

Authors

Anitha R, Murugan A
Department of Microbiology, Periyar University, Salem, Tamil Nadu, India.
Gnanendr S
Origene Biolsolutions, Anagammal Colony, Salem, Tamilnadu, India.

Abstract

The present study aimed to examine the efficacy of traditionally used herbal plant, Indigofera tinctoria in Kolli hills of Tamil Nadu against bacterial pathogen responsible for psoriasis. The plant was phytochemically screened and crude extract was prepared using solid – liquid extraction process. Then the crude extract was initially checked for in-vitro antibacterial activity at concentration of 50 mg/ml against Staphylococcus aureus by agar well diffusion method. The extract showed better activity (31 – 33 mm of zone of inhibition) compare to standard antibiotic, Tetracycline. In addition, anti-psoriatic study was carried out on Staphylococcus aureus embedded HaCat Cell line by performing the Sulphorhodamine B (SRB) assay. Based on the results, percentage activity of Indigofera tinctoria extract showed maximum inhibition of about 58.95 % at 150 μg/ml. The extracts showed potent anti-proliferant activity in HaCaT cell lines with IC50 value of 68.75±14.80 µg/ml in cytotoxicity method which is comparable to the standard drug. Medicinal plants traditionally used against psoriasis are therapeutically active against group of bacterial pathogens. Indigofera tinctoria found to be a potential candidate species for the development of novel psoriasis drugs with low cost and fewer side effects.