Antibacterial Properties of Antimicrobial peptides (AMP) from Skin Extracts of Catla catla against Wound Pathogens

Wound infection is a major complication in healing of chronic wounds. Diabetic wounds are highly prone to wound infection and development of drug resistance among pathogens has created a necessity for development of new antimicrobials for treatment of such wound infections. Antimicrobial peptides (AMP) are found to be potent source showing promising antimicrobial action. Therefore the present study focuses on extraction of AMP from Catla catla skin using two different solvents and determining antibacterial activity against wound pathogens. Specimens from wound were collected using sterile swabs and streaked on nutrient agar. Then the colonies were subjected to gram staining and series of biochemical tests for identification. Live fish of Catla catla weighing 470gm was collected, homogenized in two different solvents (Ethanol and water). Antibacterial activity of the crude extracts of AMP was evaluated by using well diffusion method. Based on the colony morphology and biochemical tests the pathogens were found to be Staphylococcus aureus and Pseudomonas aeruginosa. The wound pathogens isolated were also found to be a biofilm producer. Antibacterial analysis showed ethanolic extract had higher inhibition than aqueous extracts. Therefore the extracted AMP can be used for development of novel antibacterial drugs for treatment of drug resistant bacterial infections.