Biosynthesis of Silver Nanoparticles Using Basil Lamiaceae Leaf Extract and Screening Its Antimicrobial Activity

Authors

Hamdia, Assistant Professor, M. S. Al-Hamdani
Market Research and Consumer Protection Center, University of Baghdad, Iraq.
Sundus, Assistant Professor, Hammed. Ahamed
University of Al-Mustansiriyah, Baghdad, Iraq.

Abstract

The area of nanotechnology is the most widely used today, despite the existence of chemical and phytochemical methods because of the recent discoveries of many advanced devices for nano-scale measurements. Nanostructures are high-speed, easy-to-install and highly environmentally friendly for their low use of environmentally friendly solutions and chemicals.The synthesis of silver nanoparticles, AgNPs, was studied to demonstrate their antibacterial effect using basil leaf extract ofBasil Lamiaceae, after a qualitative evaluation of the active phytochemical components that act as anti-microbial, anti-oxidant and inhibiting blood albumin.The synthesized AgNPshave been characterized by UV-Vis spectroscopy, Scanning electron microscopy (SEM), transmission electron microscopy (TEM), andFourier-transform infrared spectroscopy (FTIR).The guide of silver nanoparticle synthesis is the color change from yellowish green to reddish brown and the surface Plasmon absorption band was measured by the UV-Visible spectroscopy.The size of the uniform spherical molecule ranged between 25 – 30 nm, the prepared nano- particle have a highest activity against pathogenic bacteria such asStaphylococcus sp., Proteuinsts sp. spseudomonas sp. Escherichiacoli. We found that nano particle appeared highest activity againstEscherichia coli followed byStaphylococcus sp, pseudomonas sp., Proteussp,respectively.