Characterization And Cytotoxicity of Novel Synthesis Selenium Nanoparticles Stabilized By Vitamin E TPGS

The aim of this research was to investigate synergistic effect of combination two related antioxidant agents by novel synthesis of selenium nanoparticles functionalized with D-α- Tocopherol polyethylene glycol 1000 succinate (TPGS) as a capping agent during chemical reduction of sodium selenite by ascorbic acid. Particle size, morphology, crystallite size of synthesized Se-TPGS were characterized by dynamic light scattering (DLS), transmission electron microscopy (TEM), Field Emission Scanning Electron Microscopy (FESEM), Ultraviolet-Visible spectrophotometry (UV/Vis), X-Ray diffraction (XRD). The in vitro antioxidant activity, cell toxicity and blood compatibility assays were investigate respectively. The DLS results revealed that the Se-TPGS possess very low polydispersity index value (PDI = 0.03) and having hydrodynamic particle size of 83.33 ± 14.7 nm with negatively value for zeta potential equal to -19.5 mV. Both SEM and TEM show regular spherical shape of SeNPs with size measurement less than DLS measuring. XRD result confirming that the produced SeNPs possessed a standard crystalline hexagonal phase structure. DPPH assay of total antioxidant activity display high antioxidant activity of Se-TPGS NPs when comparing with ascorbic acid as a reference material. The cytotoxicity of new formula Se-TPGS investigated for both cancerous (MCF-7) and normal (HBL-100) breast cell lines with significantly different. This study provides a facile and green method for chemically synthesizing stable SeNPs which are suitable for further evaluation in therapeutic applications. The SeNPs could be a blueprint in the field of biomedical application as an alternative chemotherapy for fighting cancerous cells.