Characterization And Cytotoxicity of Novel Synthesis Selenium Nanoparticles Stabilized By Vitamin E TPGS


Haider Hamzah Al-Shreefy
Department of Biochemistry, College of Medicine, Nahrain University, Baghdad, Iraq.
Mohammed J Al-Awady
Department of Genetic Engineering, College of Biotechnology. Al Qasim Green University.
Estabraq Al-Wasiti
Department of Biochemistry, College of Medicine, Nahrain University, Baghdad, Iraq.


The aim of this research was to investigate synergistic effect of combination two related antioxidant agents by novel synthesis of selenium nanoparticles functionalized with D-α- Tocopherol polyethylene glycol 1000 succinate (TPGS) as a capping agent during chemical reduction of sodium selenite by ascorbic acid. Particle size, morphology, crystallite size of synthesized Se-TPGS were characterized by dynamic light scattering (DLS), transmission electron microscopy (TEM), Field Emission Scanning Electron Microscopy (FESEM), Ultraviolet-Visible spectrophotometry (UV/Vis), X-Ray diffraction (XRD). The in vitro antioxidant activity, cell toxicity and blood compatibility assays were investigate respectively. The DLS results revealed that the Se-TPGS possess very low polydispersity index value (PDI = 0.03) and having hydrodynamic particle size of 83.33 ± 14.7 nm with negatively value for zeta potential equal to -19.5 mV. Both SEM and TEM show regular spherical shape of SeNPs with size measurement less than DLS measuring. XRD result confirming that the produced SeNPs possessed a standard crystalline hexagonal phase structure. DPPH assay of total antioxidant activity display high antioxidant activity of Se-TPGS NPs when comparing with ascorbic acid as a reference material. The cytotoxicity of new formula Se-TPGS investigated for both cancerous (MCF-7) and normal (HBL-100) breast cell lines with significantly different. This study provides a facile and green method for chemically synthesizing stable SeNPs which are suitable for further evaluation in therapeutic applications. The SeNPs could be a blueprint in the field of biomedical application as an alternative chemotherapy for fighting cancerous cells.