Development and Validation of Spectrophotometric and Chromatographic Analytical Methods of Triamterene and Hydrochlorothiazide in Dosage Form


Amol A. Raskar, Dr. Manoj S. Charde
Department of Pharmaceutical Chemistry, Government College of Pharmacy, Karad, Maharashtra, India.
Pravin P. Honmane, Akshay R. Yadav
Department of Pharmaceutical Chemistry, Rajarambapu College of Pharmacy, Kasegaon, Sangli, Maharashtra, India.


The UV and RP-HPLC method describes developed simple, rapid, specific, selective, accurate and precise method for the simultaneous estimation of triamterene and hydrochlorothiazide in dosage form. Hydrochlorothiazide diuretic agents whereas triamterene RP-HPLC method for triamterene and hydrochlorothiazide were developed using mobile phase Phosphate Buffer: Acetonitrile (70:30), flow rate 1 ml/min, injection volume 20μL, temperature 25ºC detection wavelength 222nm. Linearity range was found to be 0.66-29.8μg/ml for triamterene and 0.60-18.2μg/ml for triamterene. LOD and LOQ values were found to be 0.127 and 0.390 μg/ml for triamterene and 0.144 and 0.434 μg/ml for hydrochlorothiazide respectively. The assay result found to be 99.75% and 99.97% for triamterene and hydrochlorothiazide respectively. The developed method was validated according to ICH guidelines.