Development and Validation of Spectrophotometric Methods for Simultaneous Estimation of Cefixime Trihydrate and Linezolid in Tablet Dosage Form


Risha Wale, M S Attar
Department of Pharmaceutical Chemistry, Channabasweshwar Pharmacy College, (Degree) Latur.
Tushar Pandharkar, Tukaram M. Kalyankar
Department of Pharmaceutical Chemistry, School of Pharmacy, S. R. T. M. University, Vishnupuri, Nanded, Maharashtra, India.
Anitha K
Department of Chemistry, Sri Krishnadevaraya University, Ananthapuramu-515003, Andhra Pradesh, India.


A new, simple, rapid and novel spectrophotometric method has been developed for simultaneous estimation of cefixime trihydrate and linezolid. For this, simultaneous equation method is used. The method involved measurement of absorbance at two wavelengths, 250 nm and 286 nm, λ max of linezolid and cefixime trihydrate respectively. This method obeys Beer’s law in the employed concentration ranges of 2-12 μg/ml for both cefixime trihydrateand linezolid. The proposed method is recommended for routine analysis since it is rapid, simple, accurate, specific and also sensitive. This paper describes the development and validation of UV spectroscopic method for simultaneous estimation of cefixime trihydrate and linezolid in Tablet dosage form.