Kavitha Amirthanandam, Assistant professor, Dr. Jayachitra Ayyavoo, Assistant professor
Department of Plant Biotechnology, School of Biotechnology, Madurai Kamaraj University, Madurai, India.
Ananthi Sivagnanam, Principal
Scientist, Clinbiocare Technology, Chennai, India.
Centre of Molecular Medicine and Diagnostics (COMManD), Saveetha Dental College and Hospitals, Saveetha Institute of Medical & Technical Sciences, Chennai, India.
ELUCIDATING THE ROLE OF SAPONIN IN TREATING OVARIAN CANCER CELLS USING TWO – DIMENSIONAL ELECTROPHORESIS COUPLED WITH MASS SPECTROMETRY PROTEOMIC APPROACH
Authors
Abstract
Ovarian cancer remains a devastating disease worldwide. It affects young individuals and after that their
whole lifestyle is changed. Though many advancements are developed in the treatment strategies for
cancer, still the success rate is very low. To increase the survival rate of the affected cancer patients,
novel therapeutic strategies should be implemented. Such a novel compound is Saponin. Saponin possess
many important bioactivity functions which are used in different areas to benefit mankind. The aim of the
study to understand and evaluate the significance of Saponin in treating ovarian cancer cells. After
treating Saponin to the ovarian cancer cells, MTT analysis was performed followed by apoptotic staining.
Further to understand the protein profile changes, the protein samples were analyzed by two-dimensional
electrophoresis coupled with mass spectrometry. Interestingly identified two key candidate proteins which
are involved in apoptotic pathway namely BID and BCL2. All the analysis showed significant apoptotic
induction in the saponin treated PA1 ovarian cancer cells than untreated cells. The data clearly depicts the
significance of Saponin in treating ovarian cancer cells. This preliminary data evidently proves the impact
of Saponin for better therapeutic strategies in treating PA1 ovarian cancer cells.