Rising petroleum derivative costs and ecological contamination with their utilization have brought expanded worldwide interest in biodiesel creation and use. The journey for bio-energy in this manner has been supported all through the world to care for the climate and substitute bio-diesel with petroleum products. The goal is to assess the seed oil of cotton and to utilize it as an elective fuel. Cottonseed oil has diesel-like fuel properties. Cottonseed oil is blended in with diesel in various extents viz 5%, 10%, 15%, 20%, and 25% experimented in a compression ignition engine. Bio-diesel is generated from cottonseed through a compound interaction called transesterification. With the petrol energizes right now known and their expanding interest for utilization, it is expected that they won’t keep going long. By examination, the exhibition attributes give off an impression of being improved with the utilization of cottonseed oil for brake power, CO, HC, NO and NOx emissions.