Invitro Antagonistic Activity of a Probiotic Bacteria Enterococcus Faecalis Against White Fecal Disease Induced by Vibrio Alginolyticus of Shrimp Peneaus Monodon

Shrimp aquaculture is a normal practice in developing nations throughout Asia and the tropical world, offering non-urban communities with a means of survival and, as a consequence, poverty reduction. The white feces syndrome has caused significant economic damage in the cultured shrimp industry in China, Indonesia, Malaysia, Thailand, Vietnam and other countries in Southeast Asia. Thus, more attention should be paid to the pathogenesis of this disease. Probiotics, or beneficial bacteria that control pathogens through a variety of mechanisms, are increasingly being viewed as an alternative to antibiotic treatment. The present study was aimed to determine the probiotic activity of Enterococcus faecalis against Vibrio alginolyticus., in shrimp aquaculture. Traditional bacterial identification depended on phenotypic characteristics. Dual culture, cross streak method, and SEM analysis were used to evaluate in vitro antagonist activities. The study concludes that invitro antagonistic assay of dual culture test with Enterococcus faecalis clearly exhibited the growth inhibition of V. alginolyticus. In co-culture experiment, growth of pathogenic V. alginolyticus was inhibited by Enterococcus faecalis culture inoculated at equal ratio even in low ratio was also controlled V. alginolyticus the under invitro condition. Morphological analysis by SEM revealed that V. alginolyticus treated with Enterococcus faecalis showed major structural disruption in the cell envelope as well as a preponderance as irregular rods forms than the controls.