Isolation partial purification of fungus bio active metabolites and its antibacterial activity in pathogenic bacteria


Nelsonjoseph Lawrance, Manjudevi Mariappan, Vishnupriya Benaltraja,
Department of Biotechnology Kongunadu Arts and Science College, Coimbatore, Tamilnadu, India.
B. Vishnupriya (Corresponding author)


In the history of humanity, infectious diseases brought on by pathogenic microorganisms have been the main cause of morbidity and mortality. Few new antibiotics are being developed, despite the urgent need for them to treat infectious diseases and drug- resistant infections. Natural products are a rich source of bioactive diversity. Sample collection and purification of fungus and fungus metabolites. Identification antibacterial activity was tested against Gram-positive Staphylococcus aureus (MTCC 8708) and gram- negative Proteus vulgaris (MTCC 426), Klebsiella pneumonia (MTCC 4030), Serratia marcescens (MTCC 96). One fungi metabolite gets strong antibacterial activity against pathogenic bacteria high 30 µl of fungi metabolites produced 21 mm zone of inhibition. Selected fungi molecular identified by using ITS-1 and ITS-2 primers. Morphemically identification assess by lacto-phenol cotton blue staining.