Laboratory Practices for Purification of Polysaccharide


Ramu Govindan, Umapathi N.G, Navanita S.K
Department of Pharmacognosy, JSS College of Pharmacy, JSS Academy of Higher Education & Research, Ooty, Nilgiris, Tamilnadu, India.


The aim of the present work is to purify the mucilage polysaccharide extracted from linseed by deproteinization using Trichloroacetic acid (TCA) and Hydochloric acid (Hcl). 10% w/v TCA deproteinized more than 80% when the pH of the polysaccharide solution was 3. TCA can be considered as an better deproteinizing agent when compared to hydrochloric acid as evidenced by the highest deproteinization efficiency (88.57%).