Surgical and clinical aspects of colorectal carcinoma and their correlation with BRAFV600E expression in Iraqi patients

Authors

Dr. Suroor Abdulkareem Tarkan
M.B.Ch.B, Ministry of health and environment, Iraq.

Dr. Ihsan Ali Mohammed
Specialist surgeon, M.B.CH.B, M.R.C.S Ireland, AL-Yarmook Teaching hospital, Ministry of health and environment, Baghdad, Iraq.

Dr.Thaeer Jawad Kadhim
M.B.Ch.B, FIBMS Path, AL-Yarmook Teaching hospital, Ministry of health and environment, Baghdad, Iraq.

Abstract

background: Colorectal cancer (CRC) the most common gastrointestinal cancer in the world. In Iraq, it’s fifth of the 5 commonest cancer between males 6.33%, and the fourth of the commonest cancer in between females 4.35%. Co-morbidity, mortality, and prognosis depended on several factors like age, gender, stage, grade, general health of the patient, although the stage and grade are the most important factors. That is why different types and modalities of diagnostic tools and methods had been liberated to the field of such a serious disease to altering its progression. The oncogenic mutation in the kinase region of the BRAF gene results in abnormal cellular growth, apoptosis, resistance, tumor progression, and metastasis. Objects /To high light on surgical and clinical criteria of colonic tumors and especially focus on CRC in Iraqi patients.Also to analyze the efficacy of use immunohistochemical expression of BRAFV600E biomarker in colorectal tissues of Iraqi patients.Material and Methods/ In this study, a total of 90patients with colonic tumor referring to Al-Yarmouk Teaching Hospital, all investigations were done for the clinical, laboratory, radiology, and endoscopic with biopsy were done to them. Then after all cases with CRC (60 patients) undergone surgery each case according to the standard surgical technique he needed and specimen sent to histopathological examination, form each case 2 slides was done at a private lab. One For H&E stain and, the second one for IHC.Results/In our study the CRC is more common in old age, males, left colon, and most cases of high grade (III) and high stage (III and IV) and more than 5cm. The current study showed a correlation between BRAF