Synthesis, Characterization and Antiplatelet Activity of Antithrombotic novel 2,5-substituted aryl-7-phenyl-1,3,4-oxadiazolo-[3,2-a]-1,3,5-triazine Derivatives

Synthesized fused heterocyclic compounds 1,3,4-oxadiazolo[3,2-a]-s-triazine derivatives based on microwave mediate multi-component reaction (MCRs). In general, multi-component reactions (MCRs) are economically and environmentally very advantageous because multi-step synthesis produce considerable amounts of waste mainly due to complex isolation procedures often involving expensive, toxic and hazardous solvents after each step. Simpler procedures, equipment, lower costs, time and energy and environmentally friendly. All the synthesized compounds were characterized using physical and spectral analysis. Antiplatelet Activity of Antithrombotic synthesized compounds was carried out. A low toxicity profile of this series was observed through hemolytic, genotoxic and mutagenic assays. The most active derivatives were able to reduce both PGE2 and TXB2 production in human platelets, suggesting a direct inhibition of COX-1. These results reinforce their promising profile as lead antiplatelet agents for further in vivo experimental investigations.