Antimicrobial effected of red dragon(Hylocereus polyrhizus) on bacterial isolated from frozen meat .

Authors

Noor A. ALtaai
Unit of Zoonotic Diseases, College of Veterinary Medicine, University of Baghdad, Iraq.

Abstract

The present study pointed to estimated the validation of red dragon fruit (Hylocereus polyrhizus) extracts against three microbial pathogens (Staph. E.coli and Salmonella )isolated from frozen meat samples. One hundred twenty frozen meat samples were aseptically collected from local markets of Baghdad ,familiar bacteriological mechanism were used to isolate Staph. aureus, E. coli and Salmonella spp. Ecoli 35(29.1% ) is high percentage,Staph .aureus 21(17.5% ) and Salmonella 14( 11.6% ) isolates .The antibacterial efficiency of red dragon fruit (Hylocereus polyrhizus) extracts was determined by the agar well diffusion method to investigate the minimum inhibitory concentration (MIC) at different concentration started with 20, 10, 5, 2.5 and 1.25 mg/ml. The highest antibacterial activity between 20 mg/mL and 5 mg/mL except in Salmonella spp. the value of inhibition was between 20 mg/mL and 10 mg/ml.The highest zone of inhibition ofStaph. Auras (27 mm) While (22mm ,17mm)of E.coli and Salmonella respectively at 20 mg/mL. (21,22,12 mm) inhibition zone against Staph. aureus, E. coli and Salmonella spp. respectively at 10 mg /ml and (15, 10) mm against Staph. Aureus and E. coli at 5 mg/ml. Antimicrobial disc revealed all bacteria resistance to Bactircin, Amoxicillin clavulanic acid and Carbencillin disc, while sensitive to Tetracycline, and intermediate zone of inhibition to Ceftriaxon.