Isolation and Identification of Indole Acetic Acid (IAA) Producing Bacteria from Organic Soil: Investigating Its Efficacy on Plant Growth

Authors

Yamini P, Reshmi Gopalakrishnan
Department of Microbiology, Sri Ramakrishna College of Arts and Science for Women, Coimbatore, TamilNadu-641044, India.

Sanjay Prasad S
Department of Microbiology, CMS College of Science and Commerce, Coimbatore-641049, Tamil Nadu, India.

Karkuvelraja R
Microbial Biodiversity and Bioenergy Lab, Department of Microbiology, Bharathidasan University, Tiruchirapalli-620024, India.

Abstract

Indole acetic acid (IAA) production is a major property of rhizosphere bacteria that stimulates and facilitates plant growth. The present work deals with the isolation, characterization, and identification of indole acetic acid-producing bacteria from the rhizospheric soil. Out of four organisms isolated from serial dilution, Sample-2 confirmed thick bands of IAA production by TLC analysis. 16s rRNA analysis showed the isolated organism was found to be Pseudomonas fluorescence with a similarity of 88%. The isolated organism treat plants showed increased shoot and root length in plant growth analysis. In conclusion, the isolated IAA-producing bacteria can be used as efficient biofertilizer inoculants to promote plant growth.