Green Synthesis Of Copper Oxide Nanoparticles Using Lawsonia Inermis L. Root Characterization And Anti Cancer Potential

Medicinal plants have important place not only maintain the health and life of human beings and animals. India is the chief producer of medicinal plants and is rightly called ―Botanical Garden of the World‖. The plant Lawsonia inermis L. (family- Lythraceae) commonly known as Henna or Mehndi is known for its cosmetic properties. A preliminary qualitative phytochemical screening and anti-cancer properties of Lawsonia inermis root extracted with hydroalchol, aqueous, Ethanol, Chloroform and Petroleum ether was investigated. The bioactive compounds are rich in hydroalchol extract. Plant-mediated synthesis of nanomaterials has been increasingly gaining popularity due to its eco-friendly nature and cost-effectiveness. In the present study, we synthesized Copper oxide nanoparticles using hydro alcohol extract of root of Lawsonia inermis L. This method allowed the synthesis of nanoparticles, which was confirmed by ultraviolet-visible (UV-Vis) spectrophotometry and FTIR. UV-Vis spectra and visual observation showed that the colour of the root extracts of Lawsonia inermis L. turned into blue colour to greenish brown colour. The in-vitro anti-cancer activity in HT-29 colon cell lines was evaluated by MTT assay. The cell viability decreases while the cytotoxicity increases along with the increase in the concentration of the sample which indicates that it has potential cytotoxic effect against the colon cancer cell lines (HT-29). This plant has various medicinal properties such as antibacterial, antiviral, antimyotic, antimicrobial, anti-cancer etc. This plant is developing a innovative chemotherapeutic of pharma Industries for drug target identification.